pSG5, 4076 bp version 013001 Enzymes with 1-10 cleavage sites: #sites -- Bp position of recognition site -- AatII 1 3148 AccI 2 1, 1203 AflIII 1 1337 AhdI 1 2225 Alw44I 3 1651, 2897, 3395 AlwNI 2 965, 1748 ApaI 1 922 ApoI 8 440, 633, 641, 790, 1043 1122, 3481, 3492 AvaII 4 405, 515, 2368, 2590 BamHI 1 1049 BanI 2 2178, 3716 BanII 2 922, 3750 BciVI 2 1546, 3073 BfaI 7 352, 1157, 1210, 1832, 2085 2420, 3830 BglI 3 298, 2344, 3921 BglII 1 1055 BlnI 1 351 BpmI 1 2315 BsaAI 1 3677 BsaBI 1 1050 BsaHI 2 2766, 3148 BsaI 1 2297 BsaJI 10 63, 135, 258, 293, 302 351, 511, 970, 1497, 4030 BsaWI 3 1543, 1690, 2521 BseMII 9 9, 311, 381, 909, 1612 2021, 2187, 2727, 3389 BseRI 2 332, 570 BsiEI 5 1250, 1674, 2597, 2746, 3952 BsiHKAI 4 1651, 2812, 2897, 3395 BslI 8 253, 1353, 1371, 1537, 1816 3265, 3569, 3895 BsmAI 6 568, 997, 2297, 3062, 3216 3269 BsmBI 2 3215, 3269 BsmFI 5 60, 132, 196, 404, 498 BsmI 1 1152 Bsp120I 1 922 Bsp1286I 6 922, 1651, 2812, 2897, 3395 3750 BspHI 4 521, 2057, 3065, 3170 BspLU11I 1 1337 BsrBI 3 1268, 3069, 3821 BsrDI 2 2284, 2466 BssSI 3 1510, 2894, 3201 BstAPI 3 90, 162, 3397 BstF5I 8 216, 835, 847, 2210, 2391 2678, 3295, 3997 Cfr10I 2 2310, 3780 ClaI 1 374 Csp6I 4 673, 723, 2709, 3386 DdeI 10 9, 311, 381, 909, 1612 2021, 2187, 2727, 3153, 3389 DraI 4 638, 2094, 2113, 2805 DraIII 1 3674 DrdI 3 1439, 3309, 3628 DsaI 2 258, 511 EaeI 2 2618, 4064 EarI 3 1215, 3019, 3966 Eco57I 4 388, 726, 1864, 2912 EcoO109I 3 404, 922, 3205 EcoRI 1 1043 EcoRII 9 64, 119, 136, 838, 970 1364, 1485, 1498, 4030 FauI 7 198, 219, 3332, 3342, 3823 3877, 3960 FokI 8 216, 835, 847, 2210, 2391 2678, 3295, 3997 FspI 1 2450 HaeII 3 1581, 3826, 3834 HgaI 5 1439, 2017, 2767, 3326, 3907 HincII 3 1, 555, 1203 HinfI 10 911, 1028, 1206, 1213, 1237 1312, 1708, 2225, 3606, 3628 HphI 10 394, 508, 2081, 2308, 2704 2930, 2945, 3230, 3239, 3688 MboII 10 492, 951, 1216, 1987, 2078 2833, 2911, 3020, 3807, 3967 MslI 4 938, 2478, 2637, 2996 MspA1I 6 25, 1677, 1922, 2863, 3330 3982 MunI 1 747 MvaI 9 64, 119, 136, 838, 970 1364, 1485, 1498, 4030 NaeI 1 3780 NciI 6 920, 1716, 2412, 2763, 3265 3300 NcoI 2 258, 511 NdeI 1 3401 NgoMIV 1 3780 NsiI 2 97, 169 NspI 4 95, 167, 1337, 3255 PleI 9 911, 1028, 1206, 1213, 1237 1708, 2225, 3606, 3628 Ppu10I 2 97, 169 PpuMI 1 404 Psp1406I 3 2455, 2828, 3463 PvuI 2 2597, 3952 PvuII 2 25, 3982 RsaI 4 673, 723, 2709, 3386 SalI 2 1, 1203 SanDI 1 404 ScaI 2 672, 2708 SchI 9 911, 1028, 1206, 1213, 1237 1708, 2225, 3606, 3628 SexAI 1 118 SfcI 6 962, 1034, 1602, 1793, 2471 3898 SfiI 1 297 SmlI 4 1443, 1705, 1982, 2850 SphI 2 95, 167 SspI 4 775, 809, 3032, 3469 StuI 1 348 StyI 3 258, 351, 511 TaqI 6 2, 375, 1204, 1437, 2881 3712 TatI 4 672, 722, 2708, 3385 TfiI 1 1312 Tsp45I 5 2487, 2698, 3275, 3853, 4044 VspI 1 2401 XbaI 1 1209 XhoII 8 1049, 1055, 1978, 1989, 2075 2087, 2855, 2872 XmnI 1 2825 Enzymes that do NOT cut molecule: Acc65I AccIII AflII AgeI AscI AvaI BbeI BbsI BbvCI BclI Bpu10I Bpu1102I BsgI BsiWI BspMI BsrGI BssHII Bst1107I BstBI BstEII BstXI Bsu36I Ecl136II Eco47III Eco52I Eco72I EcoNI EcoRV EheI FseI HindIII HpaI KasI KpnI MluI MscI NarI NheI NotI NruI PacI PmeI PshAI PstI RsrII SacI SacII SapI SbfI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I Van91I XcmI XhoI XmaI