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The main topics of my research interests:
- Differential role of Ca2+-related second messengers IP3,
cADPr, and NAADP during oocyte maturation and fertilization (Lim et al., 2001,
2003; Moccia et al., 2006).
This project I carried out during my PhD training at the SZN.
This is summarised on the following pages.
- Currently I am interested in the role of Ca2+ and Ca2+ transporters in neuronal function
in health and disease.
During last few years I am mainly focused on studying the calcium homeostasis and mitochondrial dysfunction in striatal neurons
of Huntingtons Disease (Lim et al., 2007).
To study neuronal Ca2+ signaling in our Lab we use combination of the two powerful methodologies: the lentiviral gene transfer technology
and the aequorin-based Ca2+ measurement technique, which we use to study Ca2+ dynamics directly within different cell organelles,
shuch us the cytoplasm, the mitochondrial matrix, the ER lumen and others. We also use state-of-the-art tools of cell and molecular biology including primary cultures of striatal neurons
and quantitative real-time PCR.
During these years I was also involved in other two projects:
a study of mutations of the plasma-membrane calcium-pump isoform 2 causing digenic deafness
(Ficarella et al., 2007),
and the second project dealt with the control of neuronal Ca2+ homeostasis by the transcriptional repressor DREAM.
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News and Events
New Release:
Faculty of 1000
about our recent paper
Calcium homeostasis
and mitochondrial dysfunction in striatal neurons of huntington's disease. J Biol Chem 2007 Dec 21

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